The range of separation of a GPC column is determined through its porosity. Several columns can be combined to get good separation over a broad size range.
Porosity class *) | AppliChrom range | traditional | Separation Range |
---|---|---|---|
100 | 100 – 2.500 Da | 100 Å | 100 – 4.000 Da |
200 | 100 – 20.000 Da | 500 Å | 500 – 30.000 Da |
100 – 70.000 Da | 1000 Å | 500 – 60.000 Da | |
300 | 1 kDa – 300 kDa | 10e4 Å | 1 kDa – 600 kDa |
400 | 2,5 kDa – 1.000 kDa | 10e5 Å | 50 kDa – 2.000 kDa |
500 | 10 kDa – 5.000 kDa | 10 kDa – 5.000 kDa | 500 kDa – 10.000 kDa |
Screening | 100Da – 1 Mio Da |